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Pathogen DNA Source Study Underway in Wolf Run
During Spring Summer 2010 Friends of Wolf Run will be working with LFUCG, UK ERTL and Watershed Watch on "A PLAN FOR IDENTIFYING HOT-SPOTS AND AFFIRMING REMEDIATION IMPACTS ON SURFACE WATER QUALITY: PHASE I"

Samples will be analyzed for E. Coli bacteria, Total Coliform and Bacteroides qPCR (DNA) analyses to quantify human sourced E. Coli in the watershed. Contact us if you are interested in helping with the sampling on this project.

• Draft Study Project Plan (MS Word Document)
  Draft Sample Site List (MS Excel File)
  Links to photos and map of study sampling sites
  
  

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• Dissolved Oxygen Study on Wolf Run by Brandon Bell, 2009

• Purpose: To measure dissolved oxygen levels in Wolf Run Creek during the course of a day, and at the same time of day for two weeks to understand the health of the creek and it's ecosystem
  
  Download full Power Point with additional data, analysis and conclusions (768k)

• Water Quality Monitoring Data from Wolf Run
  collected by Kentucky River Watershed Watch is available at this web site.

Friends of Wolf Run completed a study with UK Environmental Research and Training Lab and Bluegrass Community and Technical College EST Program on pathogen source identification Study April - June 2008: The group sampled as part of a follow up study funded by the Kentucky Water Resources Institute through the UK ERTL Labs. This will provide follow up monitoring from a project conducted in 2007 Funded by a grant from Kentucky American Water Company)

20 stations in the watershed were sampled for E. Coli and AC/TC Ratios,

5 key sites were sampled for the presence of Somatic Coliphage viruses using EPA method 1601.

EPA Method 1601 details in this PDF document:

UK ERTL will be tested Polymerase chain reaction methods on samples from our watershed to identify genetic material of specific host types of pathogen bacteria.

Goal of the study was to determine sources high pathogen values identified as coming from human sources and to determine what health risks we face in being exposed to known high levels of pathogen bacteria in our urban streams.

Follow this link for a copy of the 2007 DNA Analysis results

Follow this link for a copy of 2008 DNA Analysis Results (PowerPoint File)

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Friends of Wolf Run participates in Stream Restoration Task Force.

Task Force Findings: (adobe PDF Document)

Collection of Stream Restoration Project Information: (File Archive, beware of large files!)

Sampling results from Fall 2005 Monitoring Project

Pathogen sample results (Adobe PDF format document)

Nutrient and Metal Sample Results (Adobe PDF format document)

Slide Show presenting findings

Get The Dirt Out "Muddy Water Watch" Campaign

Information for Streamside Landowners

Real time stream gauge on Wolf Run at Old Frankfort Pike.

US EPA Listing for Wolf Run Watershed

DRAFT Total Maximum Daily Load (TMDL) Development- Nutrients (Total Phosphorus) -For Town Branch (Fayette County, Kentucky) (PDF Document)
Technical Document on pollution issues in Town Branch watershed. (including Wolf Run)

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