Volunteer Teams Provided Materials and Instructions Sampling materials were shipped via UPS to volunteers in the project. Volunteers had already participated in a day long training workshop preparing them for this and other monitoring projects. Materials included: Sample containers Instructions for collecting field blanks (Microsoft Word Document) Instructions for collecting duplicate samples (Microsoft Word Document) General sampling instructions(Adobe PDF format document) Chain of Custody Records(Adobe PDF format document)

Registered Sampling Sites Jim Dettinger of Letcher County Kentucky collected a sample from Cram Creek. Volunteers sample pre-arranged sites on specific sampling days. The following site assignments have been made for the July 2003 sampling round. Kentucky River Sampling sites Licking River Sampling sites Salt River Sampling sites Big Sandy River Sampling sites Upper Cumberland River Sampling sites Upper Green River Sampling sites Lower Green, Tradewater River Sampling sites Four Rivers area Sampling sites

Samples Processed and collected by "runners"

Doug Epling transfers samples taken in the Perry Co. Ky area.

Don Henry of the Clark Co. Conservation District and District staff complete field testing.

Mike Mattox of the Gateway District Health Department fills out Chain of Custody records during transfer.

Updated Laboratory Contact List

• July 2003 Lab List
• Contact lab manager to make delivery arrangements for samples prior to collecting.

  Volunteer and CTI Employee Jeff Lee brings samples in to Commonwealth Technology labs in Lexington.	Lauri Holder checks in samples from Paul Russel from Pond Creek in Jefferson Co.
  Cheryl Bersaglia of Jackson County traveled from McKee to Manchester and to the lab in Lexington with her samples	Beverly Juett and Bill McGowan of Midway College Science Dept. delivering samples from Elkhorn Creek and Dix River.
  Steve Rudd of the Magoffin Co. Health Department checks his samples in at Morehead State University	Science Students from Fleming County High School deliver their samples to the Lab at Morehead State University
  Russ Barnett watches Tina Montgomery, Kevin Smith and Ralph Reiss at the University of Louisville Life Sciences Labs prepare samples for analysis	Rita from Morehead State University's Water Quality Lab runs samples from the Licking River.
  Samples are filtered and incubated in petri dishes with a specific nutrient medium for growing Fecal Coliform bacteria	Colonies grow after 24 hours and turn blue. The number of colonies are counted to get the results. How many dark blue colonies do you see on this plate?

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